sciatic nerve injury model  (Funakoshi ltd)

Journal: Polymers

Article Title: Photocrosslinkable Dexamethasone-Loaded GelMA Hydrogel for Peripheral Nerve Injury: Mechanical Behaviour and Anti-Adhesion Effect

doi: 10.3390/polym18050628

Figure Lengend Snippet: Effects of Dexa-GelMA hydrogel on anti-adhesion in a peripheral nerve injury model ( A ) Generation of a rat sciatic nerve injury model. ( B ) Representative gross images of tissue adhesion one week after hydrogel implantation in the sciatic nerve injury model. The arrows indicate the site of sciatic nerve injury site ( C ) Adhesion scoring of Nerve Tissues. *** p ≤ 0.001.

Article Snippet: In a Sprague-Dawley (SD) rat sciatic nerve injury model, implantation of the Dexa-GelMA hydrogel significantly reduced perineural adhesion and inflammation compared with the untreated control.

Techniques:

Journal: Polymers

Article Title: Photocrosslinkable Dexamethasone-Loaded GelMA Hydrogel for Peripheral Nerve Injury: Mechanical Behaviour and Anti-Adhesion Effect

doi: 10.3390/polym18050628

Figure Lengend Snippet: Effects of Dexa-GelMA hydrogel on anti-inflammation in a peripheral nerve injury model ( A ) Immunofluorescence staining of ED-1 (green) and nuclei (DAPI, blue) in injured, GelMA-, and Dexa-GelMA-treated nerves, showing reduced macrophage infiltration in the Dexa-GelMA group. ( B ) Western blot analysis of ED-1 protein expression (n = 3). ( C ) Quantitative analysis of ED-1/β-actin ratio. *** p ≤ 0.001. Scale bar 400 µm.

Article Snippet: In a Sprague-Dawley (SD) rat sciatic nerve injury model, implantation of the Dexa-GelMA hydrogel significantly reduced perineural adhesion and inflammation compared with the untreated control.

Techniques: Immunofluorescence, Staining, Western Blot, Expressing

Journal: Journal of Nanobiotechnology

Article Title: Enhancement of sciatic nerve regeneration with dual delivery of vascular endothelial growth factor and nerve growth factor genes

doi: 10.1186/s12951-020-00606-5

Figure Lengend Snippet: Illustration of peripheral sciatic nerve crush injury model

Article Snippet: Peripheral sciatic nerve crush injury model was established on Sprague Dawley (SD) rats to evaluate the therapeutic effect of PDAPEI/pDNA polyplexes.

Techniques:

Journal: Journal of Nanobiotechnology

Article Title: Enhancement of sciatic nerve regeneration with dual delivery of vascular endothelial growth factor and nerve growth factor genes

doi: 10.1186/s12951-020-00606-5

Figure Lengend Snippet: H&E staining of regenerated peripheral nerves at 4 weeks after surgery in a , e PBS group, b , f VEGF group, c , g NGF group, and d , h VEGF + NGF group. The scale bar is a – c 200 μm, and d – f 50 μm respectively

Article Snippet: Peripheral sciatic nerve crush injury model was established on Sprague Dawley (SD) rats to evaluate the therapeutic effect of PDAPEI/pDNA polyplexes.

Techniques: Staining

Journal: Journal of Nanobiotechnology

Article Title: Enhancement of sciatic nerve regeneration with dual delivery of vascular endothelial growth factor and nerve growth factor genes

doi: 10.1186/s12951-020-00606-5

Figure Lengend Snippet: TB staining of regenerated peripheral nerves at 4 weeks after surgery in a , d VEGF group; b , e NGF group; c , f VEGF + NGF group. The scale bar is a – c 200 μm; d – f 50 μm respectively

Article Snippet: Peripheral sciatic nerve crush injury model was established on Sprague Dawley (SD) rats to evaluate the therapeutic effect of PDAPEI/pDNA polyplexes.

Techniques: Staining

Journal: Journal of Nanobiotechnology

Article Title: Enhancement of sciatic nerve regeneration with dual delivery of vascular endothelial growth factor and nerve growth factor genes

doi: 10.1186/s12951-020-00606-5

Figure Lengend Snippet: Immunofluorescence staining of regenerated peripheral nerves at 4 weeks after surgery in a – d VEGF group; e – h NGF group; i – l VEGF + NGF group. a , e , i DAPI staining (blue); b , f , j BrdU labeling (green); c , g , k CD34 staining (red); d , h , l merge. The scale bar is 100 μm

Article Snippet: Peripheral sciatic nerve crush injury model was established on Sprague Dawley (SD) rats to evaluate the therapeutic effect of PDAPEI/pDNA polyplexes.

Techniques: Immunofluorescence, Staining, Labeling

Journal: PLoS ONE

Article Title: Improvement of Sciatic Nerve Regeneration Using Laminin-Binding Human NGF-β

doi: 10.1371/journal.pone.0006180

Figure Lengend Snippet: (a) Immunohistochemistry of laminin in the rat sciatic nerve. (b) Detection of laminin content in the sciatic nerve by western-blotting analysis. (c) Detection of sustained NAT-NGF and LBD-NGF at the injury sites of sciatic nerves in vivo by western-blotting analysis.

Article Snippet: To test the laminin binding ability and biological function of NAT-NGF and LBD-NGF in vivo , we used a rat sciatic nerve crush injury model. Seventy-eight male Sprague-Dawley rats weighting 200–220 g were anesthetized by an intraperitoneal injection of sodium pentobarbital (40 mg/kg body weight) and the hair on the right femur was removed.

Techniques: Immunohistochemistry, Western Blot, In Vivo